Published January 1, 2019 | Version v1
Journal article Open

Tc-99m(I) carbonyl-radiolabeled lipid based drug carriers for temozolomide delivery and bioevaluation by in vitro and in vivo

  • 1. Ege Univ, Inst Nucl Sci, Dept Nucl Applicat, TR-35100 Izmir, Turkey
  • 2. Celal Bayar Univ, Sch Med, Dept Nucl Med, Manisa, Turkey

Description

In preclinical research radiolabeled nanoparticles have been attracting interest as a new class of imaging probes. Assuming good stability of solid lipid nanoparticles (SLNs) under physiological conditions, radiolabeled SLNs can be used for imaging and measuring uptake in target tissue. Present study was performed to evaluate biological behavior of temozolomide (TMZ) loaded solid lipid nanoparticles (SLN-TMZ) in vivo and in vitro. Lipid nanoparticles were prepared by emulsification and low-temperature solidification method. zeta potential, morphology and particle size of nanoparticles were determined. Biological behavior of( 99m)Tc(CO)(3)(+) radiolabeled SLN-TMZ were investigated in vitro on U87/Daoy cell lines and in vivo on female Wistar Albino rats. Obtained results of in vitro incorporation, in vivo biodistribution and gamma imaging studies on radiolabeled SLN-TMZ show that the radiolabeled solid lipid nanoparticles could have potential as a drug delivery system for TMZ.

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