Published January 1, 2019
| Version v1
Journal article
Open
Controlled Release of Metformin Hydrochloride from Core-Shell Nanofibers with Fish Sarcoplasmic Protein
Creators
- 1. Yeditepe Univ, Fac Engn, Dept Genet & Bioengn, TR-34755 Istanbul, Turkey
- 2. Marmara Univ, Dept Biochem, TR-34854 Istanbul, Turkey
- 3. Marmara Univ, Fac Engn, Dept Chem Engn, TR-34722 Istanbul, Turkey
- 4. Marmara Univ, Fac Technol, Dept Text Engn, TR-34722 Istanbul, Turkey
- 5. Istanbul Univ Cerrahpasa, Vocat Sch Tech Sci, Dept Biomed Devices Technol, TR-34500 Istanbul, Turkey
- 6. Nanortopedi Ind & Trade Inc, Sanayi Mahallesi Teknopk Bulvari,Teknopk Istanbul, TR-34906 Istanbul, Turkey
- 7. Univ Politehn Bucuresti, Fac Appl Chem & Mat Sci, Dept Sci & Engn Oxid Mat & Nanomat, Bucharest 011061, Romania
Description
Background and Objectives: A coaxial electrospinning technique was used to produce core/shell nanofibers of a polylactic acid (PLA) as a shell and a polyvinyl alcohol (PVA) containing metformin hydrochloride (MH) as a core. Materials and Methods: Fish sarcoplasmic protein (FSP) was extracted from fresh bonito and incorporated into nanofiber at various concentrations to investigate the influence on properties of the coaxial nanofibers. The morphology, chemical structure and thermal properties of the nanofibers were studied. Results: The results show that uniform and bead-free structured nanofibers with diameters ranging from 621 nm to 681 nm were obtained. A differential scanning calorimetry (DSC) analysis shows that FSP had a reducing effect on the crystallinity of the nanofibers. Furthermore, the drug release profile of electrospun fibers was analyzed using the spectrophotometric method. Conclusions: The nanofibers showed prolonged and sustained release and the first order kinetic seems to be more suitable to describe the release. MTT assay suggests that the produced drug and protein loaded coaxial nanofibers are non-toxic and enhance cell attachment. Thus, these results demonstrate that the produced nanofibers had the potential to be used for diabetic wound healing applications.
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