Chemical constituents of Porodaedalea pini mushroom with cytotoxic, antioxidant and anticholinesterase activities

Chemical investigation of Porodaedalea pini led to the isolation of campesterol (1), ergosta-7,24(28)-dien-3 beta-ol (2), dioctyl phthalate (3), ergosterol peroxide (4), pinoresinol (5) and 4-(3,4-dihydroxyphenyl)but-3-en-2-one (6). Compounds 1, 3, and 6 were isolated for the first time from P. pini. The structures of compounds were elucidated by IR, 1D-NMR, and 2D-NMR techniques. Antioxidant, anticholinesterase, and cytotoxic activities against breast cancer cell (MCF-7) were tested. The highest antioxidant and cytotoxic activity were found in the methanol extract. Also, compound 6 was found to be active in all antioxidant tests. The hexane extract (38.15 +/- 1.50%) exhibited the highest activity against AChE enzyme while the acetone extract (48.75 +/- 0.13%) against BChE enzyme. Moreover, among isolated compounds, compound 5 was found to have the highest cytotoxic (IC50: 21.08 +/- 1.01 mu g/mL), AChE (13.73 +/- 0.85%) and BChE (80.02 +/- 0.73%) inhibitory activities. The phenolic profile was analyzed by HPLC-DAD and p-hydroxybenzoic acid (32.40 mu g/g) was identified as a major compound.