Modeling diffuse reflectance spectra of donated blood with their hematological parameters

Diffuse reflectance spectroscopy was successfully applied in transfusion medicine, for the diagnosis of the quality of blood units, in recent studies. However, removal of blood sample from the bag is still required for measuring donor dependent hematological variations in stored units. If these hematological variables are successfully interpreted from diffuse reflectance spectra, there would be no need for invasive tests. In this study, diffuse reflectance spectra of 103 erythrocyte suspensions were modeled with their donor-dependent hematological variations on the first day of storage. The final model given in this study, fitted well at 610 nm with goodness of fit of 0.94 for erythrocyte suspensions and 0.96 for 87 leukocyte-depleted units, when selecting 474 nm for normalizing diffuse reflectance spectra of each blood unit and minimizing the effects of plastic blood bag.