Electropreparation of (+/-)-10-Camphorsulfonate-Doped Poly(o-phenylenediamine) in Acetonitrile and Its Use in Determination of Glucose

Poly(o-phenylenediamine) (PoPD) film has been electrochemically prepared on Pt electrode in an acetonitrile-water medium containing o-phenylenediamine (oPD) monomer and (+/-)-10-camphorsulfonic acid (HCSA) by using the cyclic voltammetry (CV). The PoPD film (PoPD-CSA) has been characterized by FTIR, CV, EIS, FESEM, and conductivity measurement. The glucose biosensor (Pt/PoPD-CSA/GOx) has been prepared from the PoPD coated electrode by immobilizing glucose oxidase (GOx) enzyme using glutaraldehyde. The biosensor shows a low detection limit and wide linear working range, a good reusability, long-term stability, and anti-interference ability. The Pt/PoPD-CSA/GOx has possesses higher sensitivity (2.05 A/mmol L-1) and affinity to glucose due to the use of CSA ion as dopant. The linear concentration ranges of Pt/PoPD-CSA/GOx have been found to be 9.6 x 10(-3) to 8.2 mmol L-1 from calibration curve and 4.6 x 10(-2) to 100 mmol L-1 from the relationship between the (1/glucose concentration) and (1/current difference). (c) 2013 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2014, 131, 39864.