Published January 1, 2014
| Version v1
Journal article
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Molecular discrimination of Echinococcus granulosus and Echinococcus multilocularis by sequencing and a new PCR-RFLP method with the potential use for other Echinococcus species
Creators
- 1. Erciyes Univ, Dept Med Biol, Fac Med, Kayseri, Turkey
- 2. Erciyes Univ, Dept Parasitol, Fac Med, Kayseri, Turkey
- 3. Firat Univ, Fac Med, Dept Parasitol, TR-23169 Elazig, Turkey
- 4. Inonu Univ, Dept Pathol, Fac Med, Malatya, Turkey
- 5. Firat Univ, Fac Med, Dept Pathol, TR-23169 Elazig, Turkey
Description
Background/aim: To develop a novel polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) protocol using a new genomic marker sequence and a novel set of restriction enzymes in order to detect and discriminate 2 Echinococcus species, E. granulosus and E. multilocularis, found in formalin-fixed paraffin-embedded (FFPE) human tissues.
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