Published January 1, 2014 | Version v1
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Designing an Intracellular Fluorescent Probe for Glutathione: Two Modulation Sites for Selective Signal Transduction

  • 1. Bilkent Univ, Dept Chem, TR-06800 Ankara, Turkey
  • 2. Bilkent Univ, UNAM Natl Nanotechnol Res Centerand, TR-06800 Ankara, Turkey

Description

A selective probe for glutathione was designed and synthesized. The design incorporates spatial and photophysical constraints for the maximal emission signal. Thus, pHs, as well as the intracellular thiol concentrations, determine the emission signal intensity through a tight control of charge-transfer and PeT processes. The probe works satisfactorily inside the human breast adenocarcinoma cells, highlighting GSH distribution in the cytosol.

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