Published January 1, 2014
| Version v1
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Prospective Evaluation of Whole Genome MicroRNA Expression Profiling in Childhood Acute Lymphoblastic Leukemia
Creators
- 1. Ege Univ, Fac Med, Dept Pediat, TR-35100 Izmir, Turkey
- 2. Ege Univ, Fac Med, Dept Med Genet, TR-35100 Izmir, Turkey
- 3. Ege Univ, Fac Med, Dept Med Biol, TR-35100 Izmir, Turkey
- 4. Behcet Uz Childrens Hosp, Dept Pediat Hematol, TR-35210 Izmir, Turkey
- 5. Dokuz Eylul Univ, Fac Med, Dept Pediat, TR-35340 Izmir, Turkey
Description
Dysregulation of microRNA (miRNA) expression contributes to the pathogenesis of several clinical conditions. The aim of this study is to evaluate the associations between miRNAs and childhood acute lymphoblastic leukemia (ALL) to discover their role in the course of the disease. Forty-three children with ALL and 14 age-matched healthy controls were included in the study. MicroRNA microarray expression profiling was used for peripheral blood and bone marrow samples. Aberrant miRNA expressions associated with the diagnosis and outcome were prospectively evaluated. Confirmation analysis was performed by real time RT-PCR. miR-128, miR-146a, miR-155, miR-181a, and miR-195 were significantly dysregulated in ALL patients at day 0. Following a six-month treatment period, the change in miRNA levels was determined by real time RT-PCR and expression of miR-146a, miR-155, miR-181a, and miR-195 significantly decreased. To conclude, these miRNAs not only may be used as biomarkers in diagnosis of ALL and monitoring the disease but also provide new insights into the potential roles of them in leukemogenesis.
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