Published January 1, 2014 | Version v1
Journal article Open

Direct conversion of Cytochrome c spectral shifts to fluorescence using photochromic FRET

  • 1. Koc Univ, Dept Chem & Biol Engn, TR-34450 Istanbul, Turkey
  • 2. Koc Univ, Program Biomed Sci, TR-34450 Istanbul, Turkey
  • 3. Koc Univ, Dept Phys, TR-34450 Istanbul, Turkey
  • 4. Koc Univ, Dept Chem, TR-34450 Istanbul, Turkey

Description

Photochromic fluorescence resonance energy transfer (pcFRET) was used to monitor the redox activity of non-fluorescent heme protein. Venus fluorescent protein was used as a donor where its emission intensity was reversibly modulated by the absorption change of Cytochrome c.

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