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Published January 1, 2016 | Version v1
Journal article Open

Polyphenolic Composition and Evaluation of Antioxidant Activity, Osmotic Fragility and Cytotoxic Effects of Raphiodon echinus (Nees & Mart.) Schauer

Creators

  • 1. Univ Fed Santa Maria, Dept Bioquim & Biol Mol, Programa Posgrad Bioquim Toxicol, BR-97105900 Santa Maria, RS, Brazil
  • 2. Univ Fed Santa Maria, Dept Fisiol & Farmacol, BR-97105900 Santa Maria, RS, Brazil
  • 3. Univ Reg Cariri URCA, Dept Ciencias Biol, Lab Bot Aplicada, BR-63100000 Crato, CE, Brazil
  • 4. Univ Reg Cariri, Dept Quim Biol, Lab Farmacol & Quim Mol, BR-63100000 Crato, CE, Brazil
  • 5. Univ Reg Cariri, Dept Quim Biol, Lab Pesquisas Prod Nat, BR-63105000 Crato, CE, Brazil
  • 6. Univ Fed Santa Maria, Dept Farm Ind, Lab Fitoquim, BR-97105900 Santa Maria, RS, Brazil

Description

Raphiodon echinus (R. echinus) is used in Brazilian folk medicine for the treatment of inflammation, coughs, and infectious diseases. However, no information is available on the potential antioxidant, cytotoxicity and genotoxicity of this plant. In this study, the polyphenolic constituents, antioxidant capacity and potential toxic effects of aqueous and ethanolic extracts of R. echinus on human erythrocytes and leukocytes were investigated for the first time. R. echinus extracts showed the presence of Gallic, chlorogenic, caffeic and ellagic acids, rutin, quercitrin and quercetin. Aqueous and ethanolic extracts of R. echinus exhibited antioxidant activity in DPPH radical scavenging with IC50 = 111.9 g/mL (EtOH extract) and IC50 = 227.9 g/mL (aqueous extract). The extracts inhibited Fe2+ (10 M) induced thiobarbituric acid reactive substances (TBARS) formation in rat brain and liver homogenates. The extracts (30-480 g/mL) did not induce genotoxicity, cytotoxicity or osmotic fragility in human blood cells. The findings of this present study therefore suggest that the therapeutic effect of R. echinus may be, in part, related to its antioxidant potential. Nevertheless, further in vitro and in vivo studies are required to ascertain the safety margin of its use in folk medicine.

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