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Published January 1, 2016 | Version v1
Journal article Open

HPLC-DAD phenolic profile, cytotoxic and anti-kinetoplastidae activity of Melissa officinalis

Creators

  • 1. Univ Reg Cariri, Dept Quim Biol, Lab Microbiol & Biol Mol, Crato, CE, Brazil
  • 2. Fdn Moises Bertoni, Ctr El Desarrollo Invest Cient CEDIC, Lab Diaz Gill, Asuncion, Paraguay
  • 3. Univ Fed Santa Maria, Ctr Ciencias Nat & Exatas, Dept Bioquim & Biol Mol Bioquim Toxicol, BR-97105900 Santa Maria, RS, Brazil
  • 4. Univ Fed Pampas, Sao Gabriel, RS, Brazil

Description

ContextMelissa officinalis subsp. inodora Bornm. (Lamiaceae) has been used since ancient times in folk medicine against various diseases, but it has not been investigated against protozoa.Objective To evaluate the activities of M. officinalis against Leishmania braziliensis, Leishmania infantum and Trypanosoma cruzi as well as its cytotoxicity in fibroblast cell line.Materials and methods The fresh leaves were chopped into 1cm(2) pieces, washed and macerated with 99.9% of ethanol for 72h at room temperature. Antiparasitic activity of M. officinalis was accessed by direct counting of cells after serial dilution, while the cytotoxicity of M. officinalis was evaluated in fibroblast cell line (NCTC929) by measuring the reduction of resazurin. The test duration was 24h. High-performance liquid chromatography (HPLC) was used to characterise the extract.Results The extract at concentrations of 250 and 125g/mL inhibited 80.39 and 54.27% of promastigote (LC50 value=105.78g/mL) form of L. infantum, 80.59 and 68.61% of L. brasiliensis (LC50 value =110.69g/mL) and against epimastigote (LC50 value =245.23g/mL) forms of T. cruzi with an inhibition of 54.45 and 22.26%, respectively, was observed. The maximum toxicity was noted at 500g/mL with 95.41% (LC50 value=141.01g/mL). The HPLC analysis identified caffeic acid and rutin as the major compounds.Discussion The inhibition of the parasites is considered clinically relevant (<500g/mL). Rutin and caffeic acids may be responsible for the antiprotozoal effect of the extract.Conclusion The ethanol extract of M. officinalis can be considered a potential alternative source of natural products with antileishmania and antitrypanosoma activities.

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