Published January 1, 2016
| Version v1
Journal article
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ENHANCED beta-MANNANASE PRODUCTION FROM ALTERNATIVE SOURCES BY RECOMBINANT ASPERGILLUS SOJAE
Creators
- 1. Akdeniz Univ, Fac Eng, Dept Food Eng, Dumlupinar Blvd, TR-07058 Antalya, Turkey
- 2. Alanya Hamdullah Emin Pasa Univ, Fac Tourism, Dept Gastron & Culinary Arts, Cikcilli Beldesi Saraybeleni Area, Saraybeleni St 7, TR-07400 Antalya, Turkey
- 3. Kocaeli Univ, Ctr Stem Cell & Gene Therapies Res & Practice, Kocaeli Cayirova Rd, TR-041380 Kocaeli, Turkey
- 4. Middle E Tech Univ, Univ Area, Fac Eng, Dept Food Eng, Dumlupinar Blvd 1, TR-06800 Ankara, Turkey
Description
beta-mannanases can degrade galactomannans to mannose and it has been used in various application areas. The aim of this study was to produce the beta-mannanase from carob pod extract including different nitrogen sources. The best operation values for fermentation were determined to be 8% initial sugar concentration with 0.5% yeast extract, 100 r.p.m., and 7% inoculation rate, which yielded the maximum beta-mannanase activity as 423.60 U ml(-1). Effects of nitrogen sources on beta-mannanase activity were also studied and it reached 695.6 U ml(-1) by using 0.5% of ammonium nitrate as the nitrogen source at the determined optimum conditions. Results also showed that meat bone meal and soybean meal could be used as cost effective nitrogen sources based on achieved beta-mannanase activity.
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