Yayınlanmış 1 Ocak 2017 | Sürüm v1
Dergi makalesi Açık

Reversible Light-Switching of Enzymatic Activity on Orthogonally Functionalized Polymer Brushes

  • 1. Paul Scherrer Inst, Lab Micro & Nanotechnol, CH-5232 Villigen, Switzerland
  • 2. Bogazici Univ, Dept Chem, TR-34342 Istanbul, Turkey
  • 3. ETH, Dept Mat, Lab Surface Sci & Technol, CH-8093 Zurich, Switzerland

Açıklama

maleimide-containing monomer, were grafted from radical initiators at the surface of irradiation-actiyated fluoropolymer foils. After postpolymerization modification with enzymatically active microperoxidase-11 and photochromic spiropyran moieties, the polymer brushes catalyzed the oxidation,of 3,3'5,5'- tetramethylbenzidine. Exposure to either UV or visible-light allowed switching the turnover by more than 1 order of magnitude, as consequence of the reversible, light-induced spiropyran-merocyanine transition. The modified samples were integrated into an optofluidic device that allowed the 'reversible switching of enzymatic activity for several cycles under flow, validating the potential for application in smart lab-on-a-chip systems.

Dosyalar

bib-c1dab871-ad5f-4b6f-bfef-81b81d8490f1.txt

Dosyalar (233 Bytes)

Ad Boyut Hepisini indir
md5:a4c95cea4ca2b3bfe68450d000b2c987
233 Bytes Ön İzleme İndir