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Published January 1, 2017 | Version v1
Journal article Open

SAFETY ASSESSMENT AND ANTIOXIDANT ACTIVITY OF LANTANA MONTEVIDENSIS LEAVES: CONTRIBUTION TO ITS PHYTOCHEMICAL AND PHARMACOLOGICAL ACTIVITY

Creators

  • 1. Univ Fed Santa Maria, Dept Bioquim & Biol Mol, Programa Posgrad Bioquim Toxicol, BR-97105900 Santa Maria, RS, Brazil
  • 2. Univ Fed Santa Maria, Dept Farmacol, Programa Posgrad Ciencias Farmceut, BR-97105900 Santa Maria, RS, Brazil
  • 3. Fdn Moises Bertoni, CEDIC, Lab Diaz Gill, Asuncion, Paraguay
  • 4. Univ Reg Cariri URCA, Dept Quim Biol, Lab Farmacol, Crato, Ceara, Brazil
  • 5. Univ Reg Cariri URCA, Dept Quim Biol, Lab Pesquisas Prod Nat, BR-63105000 Crato, Ceara, Brazil
  • 6. Univ Fed Santa Maria, Dept Farm Ind, Lab Fitoquim, BR-97105900 Santa Maria, RS, Brazil
  • 7. Univ Peshawar, Inst Chem Sci, Peshawar 25120, Khyber Pakhtunk, Pakistan
  • 8. Univ Fed Rio Grande do Sul, Inst Ciencias Basic Saude, Dept Bioquim, BR-90035003 Porto Alegre, RS, Brazil

Description

Lantana camara, the widely studied species, and L. montevidensis, the less studied species of the genus Lantana are both used in traditional medicine for the same purpose (anti-asthma, anti-ulcer, anti-tumor, etc). However, little is known about the toxicity of L. montevidensis and there is limited information on its chemical constituents. Here, we investigated for the first time the genotoxicity and cytotoxicity of the ethanolic (EtOH) and aqueous extracts from the leaves of Lantana montevidensis in human leukocytes, as well as their possible interaction with human erythrocyte membranes in vitro. The antioxidant activities of both extracts were also investigated in chemical and biological models. Treatment of leukocytes with EtOH or aqueous extracts (1-480 mu g/mL) did not affect DNA damage index, but promoted cytotoxicity at higher concentrations (240-480 mu g/mL). Both extracts did not modify the osmotic fragility of human erythrocytes. The extracts scavenged DPPH radical and prevented Fe2+-induced lipid peroxidation in rat's brain and liver homogenates, and this was likely not attributed to Fe (II) chelation. The HPLC analysis of the extracts showed different amounts of polyphenolic compounds (isoquercitrin, gallic acid, catechin, ellagic acid, apigenin, kaempferol, caffeic acid, rutin, quercitrin, quercetin, chlorogenic acid, luteolin) that may have contributed to these effects. These results supported information on the functional use of L. montevidensis in folk medicine.

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