Published January 1, 2017
| Version v1
Journal article
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Three-dimensional cell culture of human mesenchymal stem cells in nanofibrillar cellulose hydrogels
Creators
- 1. Univ Reading, Sch Pharm, Stem Cell Biol & Regenerat Med Grp, Whiteknights Campus, Reading RG6 6AP, Berks, England
- 2. Lein Appl Diagnost Ltd, Reading Enterprise Ctr, Whiteknights Rd, Reading RG6 6BU, Berks, England
- 3. Univ Reading, Sch Biol Sci, Cell Migrat Lab, Whiteknights Campus, Reading RG6 6AP, Berks, England
- 4. Selcuk Univ, Fac Dent, Dept Periodontol, TR-42079 Konya, Turkey
Description
Human mesenchymal stem cells (MSCs) are the most intensely studied and clinically used adult stem cell type. Conventional long-term cultivation of MSCs as a monolayer is known to result in a reduction of their functionality and viability. In addition, large volumes of cell culture medium are required to obtain cell quantities needed for their clinical use. In this proof of concept study, we cultivated human MSCs within a three-dimensional nanofibrillar cellulose (NFC) hydrogel. We show that NFC is biocompatible with human MSCs, and represents a feasible approach to upscaling of their culture.
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