Published January 1, 2009 | Version v1
Journal article Open

Immunoproteomic analysis of Bordetella pertussis and identification of new immunogenic proteins

  • 1. Middle E Tech Univ, Dept Biol Sci, TR-06531 Ankara, Turkey
  • 2. Vaccine Biol Res Co, Ankara, Turkey
  • 3. Ernst Moritz Arndt Univ Greifswald, Inst Mikrobiol, D-17487 Greifswald, Germany

Description

Bordetella pertussis is the causative agent of highly communicable respiratory infection whooping cough (pertussis) which remains one of the world's leading causes of vaccine-preventable deaths. In the present study, total soluble proteins extracted from two B. pertussis strains, Tohama I and the local isolate Saadet were separated by two-dimensional gel electrophoresis and analyzed by Western blotting for their reactivity with the antisera obtained from the mice immunized with inactivated whole cells as well as those collected from the mice challenged intraperitoneally with live cells of each strain. Of a total of 25 immunogenic proteins identified, 21 were shown to be the novel antigens for B. pertussis. (C) 2008 Elsevier Ltd. All rights reserved.

Files

bib-dbf0779f-bcf2-4fc1-af46-19202e4f0a8b.txt

Files (220 Bytes)

Name Size Download all
md5:2dd54543c03f02220cb4358b96b28a2f
220 Bytes Preview Download