In vitro release and cytotoxicity of cisplatin loaded methoxy poly (ethylene glycol)-block-poly (glutamic acid) nanoparticles against human breast cancer cell lines

Different formulations of methoxy poly (ethylene glycol)-block-poly (glutamic acid) (mPEG-b-PLG) were successfully synthesized. Drug loading, in vitro release and cytotoxicity of Cis-Diaminodichloro platinum (II) (CDDP, cisplatin) loaded nanoparticles were investigated. The synthesized polymers were structurally characterized by Proton Nuclear Magnetic Resonance (H-1 NMR) and Fourier Transform Infra-Red (FTIR). Number average molecular weight (Mn), degree of polymerization (DP) and poly dispersity index (PDI) were determined. Drug loading contents and drug loading efficiency were quantified. The drug loaded nanoparticles were developed in small size, uniform shape and with slightly negative zeta potential. There was more release at lysosomal pH (5.5) as compared to physiological pH (7.4). The release was in a sustained and controlled manner which was consistent with the in vitro longevity of the nanoparticles. The effect of glutamic acid moieties on in vitro release was also investigated. There was dose and time dependent cell proliferation inhibition of the free drug and drug loaded nanoparticle. The biocompatibility, optimum size, shape and surface charge of the developed nanoparticles make them an efficient drug delivery carrier. (c) 2017 Elsevier B.V. All rights reserved.