Published January 1, 2018
| Version v1
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Investigation of Hepatitis A and E Viruses in Mussels Collected from the Bosphorus, in Istanbul, Turkey - Short Communication
Creators
- 1. Istanbul Univ, Vet Fac, Dept Virol, Istanbul, Turkey
- 2. Firat Univ, Vet Fac, Dept Food Hyg & Technol, Elazig, Turkey
- 3. Istanbul Univ, Vet Fac, Dept Food Hyg & Technol, Istanbul, Turkey
- 4. Adnan Menderes Univ, Dept Food Hyg & Technol, Istanbul, Turkey
- 5. Univ Ankara, Vet Fac, Dept Food Hyg & Technol, Ankara, Turkey
- 6. Ctr Lab, Biruni Labs, Istanbul, Turkey
- 7. Istanbul Univ, Cerrahpasa Fac Med, Dept Microbiol, Istanbul, Turkey
- 8. Cent Vet Inst, Lelystad, Netherlands
- 9. Kansas State Univ, Coll Vet Med, Dept Diagnost Med & Pathobiol, Manhattan, KS 66506 USA
Description
Hepatitis A and E viruses (HAV and HEV) are recognised as food-and water-borne infections and shellfish are a well-documented source of these viruses. The presence of HAV and HEV in mussels has not previously been investigated in Bosphorus, Istanbul, Turkey, and therefore the aim of this study was to investigate the frequency of HAV and HEV in mussels collected from this location. A total of 92 pooled samples representing 736 mussels originating along the Bosphorus coast were collected from fish distributors. RNA was extracted using the RNeasy Plant Mini Kit and a TaqMan real-time RT-PCR was performed using primers specific to HAV and HEV. Amongst the 92 pooled samples tested, three (3.3%) were found to be positive for HAV by the TaqMan real-time RT-PCR. No HEV was detected in any of the mussels tested. A 174 bp product was observed on gel electrophoresis with HAV positive samples detected by SYBR Green real-time PCR after reverse transcription. This product was sequenced and confirmed to contain HAV sequences by alignment using the data in GenBank. This is the first report describing the presence of HAV in mussels harvested in the Bosphorus, Istanbul, Turkey, indicating the potential contamination of aquatic environment and risk to public health.
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