Bioactive Compounds From <i>Rosa canina</i> L. Roots With Anti-Collagenase, Anti-Tyrosinase, and Antioxidant Activities: In Vitro and In Silico Approaches

The study aimed to investigate the anti-aging, skin-whitening, and antioxidant capacities of the extracts and constituents from the roots of Rosa canina L. by anti-collagenase, anti-tyrosinase activity, ferric-reducing antioxidant power (FRAP), cupric-reducing antioxidant capacity (CUPRAC) assays, and the Folin-Ciocalteu method (for the extracts). Enzyme inhibitory activities, pharmacokinetics, and drug-likeness properties of the compounds were evaluated in silico. According to the results of activity studies, the ethyl acetate subextract exhibited the highest anti-tyrosinase activity, and the remaining aqueous subextract demonstrated the highest anti-collagenase activity. The isolation studies led to the purification of euscaphic acid (1), tormentic acid (2), kaji-ichigoside F1 (3), rosamultin (4), 2-oxopomolic acid (5), and (+)-catechin (6) for the first time from the roots of this plant. Isolated compounds exhibited anti-collagenase and anti-tyrosinase activities. The extracts and (+)-catechin had higher antioxidant capacities than other compounds. R. canina roots and their compounds can be included in dermocosmetic products after further studies.