Electrodeposition of carbon nanotubes and conjugation of arginyl-glycyl-aspartic acid for the following of glioblastoma cells on bionanocomposites

The improvement of surface treatment methods that permit the tuning of cell adhesion on the surface of biomaterials and devices is of considerable importance. Here, multi-walled carbon nanotubes (MWCNT) were modified with 4-aminothiophenol (4ATP). Then, electrodeposition of MWCNT-4ATP was carried out on 4ATPmodified screen-printed gold electrodes (SP-Au). After conjugation of Arginyl-glycyl-aspartic acid (RGD)-pep- tide on Poly(MWCNT-4ATP), the adhesion of U-87MG glioblastoma cells was examined by differential pulse voltammetry (DPV) technique. The synthesized MWCNT-4ATP and the obtained Poly(MWCNT-4ATP)/RGD surfaces were characterized using Scanning Electron Microscopy-Energy Dispersive X-Ray Spectrometer (SEMEDS), Attenuated Total Reflection Fourier Transform Infrared Spectroscopy (ATR-FTIR), X-Ray Photoelectron Spectrometer (XPS). The linear range for U-87MG glioblastoma cells was 102-106 cells/mL. The developed Poly (MWCNT-4ATP)/RGD cell adhesion platform provided monitoring of U-87MG glioblastoma cells using DPV technique and fluorescent imaging.