Karyoevolutive consideration of two sea fish species via the newest short-term culture method

This study successfully determined the chromosome numbers and karyotypes of two marine fish species from separate families using the short-term culture method (PB-MAXTM application). The culture incubation periods varied for each species. Kidney tissues from the fish samples were treated with PB-MAXTM for either 2.5 or 4 h. Results showed that diploid chromosome numbers, karyotypes, and arm numbers differ by species: Red Mullet, Mullus barbatus, has 2n-44, with 3 pairs of metacentric, 7 pairs of submetacentric, 7 pairs of subtelocentric, and 5 pairs of acrocentric chromosomes (NF=64). Round Goby, Neogobius melanostomus, has 2n-46, consisting of 46 pairs of acrocentric chromosomes (NF=46). C-and NOR-positive results were achieved through constitutive heterochromatin banding and silver nitrate staining for both species. The primary objective of this research is to demonstrate the applicability of shortterm culture in producing successful cytogenetic results in marine fish. This study will support future research and facilitate chromosome studies on marine fish, making the process quick and practical.