A Novel DLLME-Based Approach for the Spectrophotometric Determination of Mercury in Environmental Samples Using the Fe(II) Phthalocyanine Sensor

In the present investigation, a novel dispersive liquid-liquid microextraction (DLLME) method was developed for the spectrophotometric determination of Hg2+. Fe(II) phthalocyanine (Fe(II)Pc) was employed as the sensor, chloroform (300 mu L) as the extraction solvent, and ethanol (700 mu L) as the dispersive solvent. Following the formation of the Hg2+:Fe(II)Pc complex, the sample was centrifuged at 1000 rpm for 2 min. The aqueous phase was discarded, and the extraction phase was diluted to 250 mu L with methanol and transferred into a 250 mu L quartz cell for spectrophotometric measurement at 276 nm. The method exhibited a linear range of 1-20 mu g/L, with limits of detection (LOD) and quantification (LOQ) calculated as 1.44 mu g/L and 4.80 mu g/L, respectively. The enrichment factor was determined to be 105, and the optimum pH for the procedure was 2.0.