Published January 1, 2018 | Version v1
Journal article Open

A novel multiplex PCR tool for simultaneous detection of three root-knot nematodes

  • 1. Univ Akdeniz, Dept Plant Protect, Fac Agr, TR-07058 Antalya, Turkey
  • 2. Bati Akdeniz Agr Res Inst, TR-07100 Antalya, Turkey
  • 3. Mugla Sitki Kocman Univ, Dept Mol Biol & Genet, Fac Sci, TR-48000 Mugla, Turkey

Description

Root-knot nematodes, Meloidogyne incognita, M. javanica and M. arenaria, cause serious yield losses in protected growing areas in Turkey. They also show a'co-infection' profile in fields' soil microflora. In this study, we designed multiplex primers for detecting all three species simultaneously. Additionally, the confidence and definition of this method were tested by using mixed and large populations related to these species at different biological stages. We suggest this effective tool be used to easily identify three root-knot nematodes that will be beneficial for designing crop rotation modelling in protected growing areas in Turkey as well as quarantine requirements.

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