Dataset for: Integrated SELEX Monitoring via a Normalized Enrichment Index: A Workflow-Based Proof-of-Concept Study

This dataset provides the supplementary and processed data supporting the manuscript titled "Integrated SELEX Monitoring via a Normalized Enrichment Index: A Workflow-Based Proof-of-Concept Study".

The repository contains three data files: 1. Sequence Length Distribution: Summary of high-throughput sequencing (HTS) read length distributions across selected SELEX rounds (S2–S12). 2. Sequence Frequencies & Tracking IDs: Read counts and Reads Per Million (RPM) data for the Top 100 aptamer clones. Note: Full nucleotide sequence strings have been masked and replaced with unique tracking codes (e.g., A1–A100) to protect proprietary sequence information pending further functional characterization. 3. qPCR Melt Curve Analysis: Raw, processed, and normalized fluorescence derivative (-dF/dT) data demonstrating the thermal stability shifts and structural evolution of the aptamer pools.

These datasets validate the pool-level enrichment trends and support the utility of the Normalized Enrichment Index (NEI) proposed in the study.