Development of electrochemical 3-MCPD sensor based on molecularly imprinted polymer coating on metal organic framework modified gold electrode

3-Chloropropane-1,2-diol (3-MCPD) is formed during the food processing and defined as a potential carcinogen. For this reason practical, effective and economical detection of this substance is crucial. In this work, Fe-MIL-88metal organic framework (MOF) included electrochemical molecularly imprinted polymer (MIP) based sensor was designed for 3-MCPD detection. Gold screen printed electrodes (AuSPE) were modified with MOF structure and then a MIP layer was formed on the electrode surface via electropolymerization where aniline was used as a monomer and 3-MCPD as a template molecule. In order to improve the response of the prepared sensor, various experimental parameters such as MOF amount, MIP layer thickness, template molecule: monomer ratio, extraction time and rebinding time were optimized. Then, as an analytical characteristic parameter, linear ranges were detected for 3-MCPD in the range of 0.05 mu M to 0.5 mu M with a limit of detection (LOD) value of 0.43 mu M as well as in the range of 0.05 and 0.5 mu M with LOD value of 0.01 mu M. The specificity of the developed electrochemical sensor was investigated in the presence of interferent reagents and then developed sensor was adapted for 3-MCPD determination in vegetable oil and soy sauce. Lastly the real sample analysis was also validated with gas chromatograpy-mass spectrometry technique which is accepted as gold standard method for 3-MCPD analysis.