Research Article Phage Isolation, Characterization and Antibiofilm ActivityAgainst Multidrug-Resistant Pseudomonas aeruginosa

Background and Objective: Biofilms are structured microbial communities that occur as surface-attached communities aggregates. By penetrating the biofilm matrix and infecting bacterial cells, bacteriophages can disrupt the structure of the biofilm. Therefore, bacteriophages can be used as an alternative strategy in the control and pathogenic bacteria that form antibiotic-resistant biofilms. Factors such as the specificity of bacteriophages, infection persistence, mechanism of action and their ability to penetrate bacterial cells within the biofilm need better understanding. This study may contribute to developing new therapeutic options and reveal the potential of bacteriophages in the treatment of multidrug-resistant Pseudomonasaeruginosa infections. Materialsand Methods: The double layer agar technique was used to isolate bacteriophage.The phage was characterized using a transmission electron microscope.The host range of the phage as well as its antimicrobial and antibiofilm properties were determined. The antibiofilm effect of the bacteriophage was evaluated using crystal violet method a 96-well flat-bottom microplate setup. Results: Clear results were obtained for 5 isolates on 10 bacterial plates with strong biofilm ability. It was observed that the isolated bacteriophage has a wide thermal range and does not lose its infectivity at different pH levels. Bacteriophages showed an average antibiofilm effect of 82 and 59% at varying bacterial dilutions of 1/100 and 1/1000, respectively. Conclusion: It was observed that it showed a stronger antibiofilm effect against the isolate with strong biofilm formation ability than the control. However, further research is needed to assess the anti-biofilm effects of bacteriophages. In general, the results suggest that the bacteriophage isolated may be a strong antibiofilm candidate.