The effect of anthocyanin administration on redox balance in acute exercise: an experimental study

Purpose: Due to the diverse biological activities attributed to its antioxidant, antidiabetic, anti-inflammatory, and cytoprotective properties, cyanidin-3-glucoside (Cy3G) has been widely studied. The present study was conducted to investigate the effects of Cy3G supplementation on redox balance disruption during acute exercise. Materials and Methods: To this end, 28 male Balb-C mice were divided into four groups: control, exercise, Cy3G, and exercise+Cy3G. During the experimental period, the mice in the Cy3G and exercise+Cy3G groups were administered 5 mg of Cy3G per kg of body weight, while the control and exercise groups were fed standard chow. The mice were trained on treadmill for 10 min every day at speed of 10 m/min for 2 weeks. On the 15th day, an acute exhaustion exercise was applied. Then, all groups were sacrificed, and serum samples were taken to analyze the native thiol, total thiol, disulfide, and thiol-disulfide indices. Results: The findings showed that the combination of Cy3G and exercise significantly increased native thiol levels and decreased disulfide levels compared to the other groups. However, it did not cause any change in total thiol levels. Native thiol were measured as 114.6 +/- 64.1, 106.4 +/- 57.9, 200.1 +/- 84.2, 262.1 +/- 105.9; Disulfide were determined as 253.2 +/- 71.6, 257.4 +/- 31.5, 213.8 +/- 44.8 and 188.4 +/- 32.2; Total thiol were 621.1 +/- 116.1, 621.2 +/- 100.1, 627.7 +/- 125.8 and 639.0 +/- 105.3 (respectively; control, exercise, Cy3G, and exercise+Cy3G). Conclusion: Cy3G supplementation can potentially maintain redox balance during acute exercise by supporting antioxidant defense systems and reducing oxidative stress. The use of Cy3G may have a positive effect on preventing exercise-induced oxidative stress and acute fatigue.