Phenolic Profile, Bioactivities and <i>In Silico</i> Analysis of the Trunk Bark of <i>Acacia Cyanophylla</i> Lindl.

In the current investigation, total phenolics and flavonoids of the methanolic extract obtained from the trunk bark of Acacia cyanophylla Lindl. were quantified by LC-HRMS technique. DPPH and ABTS reagents were employed to assay the antioxidant potential. The anti-tyrosinase and anti-alpha-amylase potentials were also assayed. The findings revealed that thirteen polyphenolic compounds were detected in the methanolic extract with trans-taxifolin (23.2 g/kg), as the major constituent. A. cyanophylla extract displayed a higher activity with DPPH test (IC50=10.14 +/- 1.00 mu g/mL) than with ABTS (IC50=15.27 +/- 2.09 mu g/mL). The same extract also exhibited interesting alpha-amylase inhibitory action (IC50 value of 4.00 +/- 0.17 mu g/mL). Moreover, methanolic trunk bark extract exerted strong anti-tyrosinase capacity with an IC50 of 5.12 +/- 0.41 mu g/mL in comparison to kojic acid (IC50=10.22 +/- 0.85 mu g/mL) used as positive control. The antioxidant, anti-tyrosinase and anti-alpha-amylase potentials of the methanolic extract of A. cyanophylla trunk bark were reinforced by in silico molecular docking analyses, which confirmed the results of the in vitro tests.