Determination of carcinoembryonic antigen (CEA) by surface plasmon resonance-enhanced total internal reflection ellipsometry (SPRe-TIRE)
Creators
- 1. Alanya Alaaddin Keykubat Univ, Rafet Kayis Fac Engn, Fundamental Sci, Antalya, Turkiye
- 2. Bilecik Seyh Edebali Univ, Fac Engn, Dept Bioengn, Bilecik, Turkiye
- 3. Kutahya Dumlupinar Univ, Fac Arts & Sci, Dept Chem, Kutahya, Turkiye
- 4. Necmettin Erbakan Univ, Ahmet Kelesoglu Fac Educ, Chem Dept, Konya, Turkiye
Description
A surface plasmon resonance-enhanced total internal reflection ellipsometric (SPRe-TIRE) aptasensor is reported for the determination of the carcinoembryonic antigen (CEA). A gold nanofilm-coated SF10 glass slide was modified with electrochemical diazonium salt reduction and gold nanoparticles (AuNPs) to form a sensor. Next, a specific aptamer for CEA was immobilized on the AuNPs via self-assembly. The platform was characterized by X-ray photoelectron spectroscopy, ellipsometry, and cyclic voltammetry for each step of the preparation of the sensor. CEA aptamers with different affinities were used. SPRe-TIRE employed total internal reflection geometry and AuNPs to enhance plasmon resonance for the sensitive and selective determination of CEA in human serum. The working range of the aptasensor was from 0.01 to 500 ng/mL with an optimum detection limit of 0.1 pg/mL.
Files
bib-5a88fbb1-089a-40a9-9919-172117e1d795.txt
Files
(277 Bytes)
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