Non-invasive electrochemical immunosensor for reverse iontophoretic determination of cardiac troponins (cTnT & cTnI) in a simulated artificial skin model. Significance of raw DPV and CV data for chemometric discrimination

Electrochemical immunosensors coupled with reverse iontophoresis (RI) for noninvasive determination of cardiac troponins were developed and validated according to ICH Q2 (R1) guideline. Linearity was in 0.01-10 and 100-500 ng/mL ranges. LODs (ng/mL) were in 6-25 x 10-4, while LOQs (mu g/mL) were in 18-7.5 x 10-4 range. Chemometric evaluation was performed on raw data simply by principle component analysis and cluster analysis to discriminate stages of immunosensors. This is the first demonstration of RI determination of cardiac troponins so far. Findings of the current manuscript have great potential to develop point of care diagnostic systems for major cardiac events, where high sensitivity and specificity are required.