Micropropagation Protocols of New <i>Prunus</i> Hybrids with Significant Rootstock Potential in Fruit Breeding and Cultivation

In vitro micropropagation is the most valid production method used for disease-free, clonal, and mass production of plants developed using unique breeding methods. This study was carried out to establish appropriate protocols for the micropropagation of 10 (Prunus amygdalus) x (P. amygdalus x P. persica) hybrid rootstocks obtained by crossbreeding. The maximum micro shoot and shoot length values were obtained from the medium (MED 11) as 3.92 shoots/explant and 14.59 mm, respectively. The best rooting values were obtained from MS medium supplemented with 1.75 mgL(-1) IBA + 0.1 mgL(-1) BAP (MED-5) in the rooting study. The highest average root number, root length, and root diameter of shoots 2.79 pcs/explant, 14.36 mm, and 1.15 pcs/explant, respectively, were observed. It is suggested that MS media supplemented with MED-11 (0.1 mg.L-1 IBA + 1.75 mg.L-1 BAP + 0.2 mg.L-1 GA(3)), MED-12 (0.1 mg.L-1 IBA + 2.0 mg.L-1 BAP + 0.2 mg.L-1 GA(3)) for regeneration, MED-5 (1.75 mg.L-1 IBA + 0.1 mg.L-1 BAP), and MED-6 (2.0 mg.L-1 IBA + 0.1 mg.L-1 BAP) hormone combinations for rooting would be appropriate for the micropropagation of hybrid rootstocks. It is thought that the results of the study can be a guide for new studies to be carried out especially in these species.