<i>In Vitro</i> Evaluation of Anti-Angiogenic Efficacy, Cell Migration Activity and Cell Survival Effect Against Hydrogen Peroxide-Induced Damage of Prunetin

Anti-angiogenic isoflavonoids are gaining attention as a novel approach in the prevention and treatment of tumor progression and metastasis. These phenylpropanoid compounds as bioactive phytoestrogens exhibit various pharmacological activities including antimicrobial, anti-inflammatory and antioxidant effects. The purpose of this study was to evaluate the anti-angiogenic, antioxidant and cell migration effects of prunetin in in vitro studies. The antioxidant activity of prunetin was performed against H2O2-induced cell damage on Vero cells. The cell migration effect was comparatively studied with HaCat cells. The anti-angiogenic potential of prunetin was assessed using an in vitro HET-CAM model. The strongest anti-angiogenic effect of prunetin was observed at 100 and 200 mu M on the chorioallantoic membranes of hen eggs when compared to the control group. Similarly, the statistical analysis of data showed that prunetin treatment at 200 mu M significantly induced cell migration with a ratio of 98.65 +/- 0.46% on HaCaT cells. Additionally, the excessive protective effect of prunetin against H2O2-induced cytotoxicity in Vero cells was shown with 90% cell viability at the highest concentration. In conclusion, prunetin can stimulate keratinocytes migration and can induce antioxidant mechanisms which involves direct inhibition of the ROS generation and anti-angiogenic responses. It is suggested that prunetin can be applied to support anti-cancer treatment given its promising results since isoflavonoids possess antioxidant, anti-inflammatory and cell migration properties.