Boron nitride nanosheet modified amperometric biosensor for uric acid determination

In this study, enzyme-modified boron nitride nanosheets as a catalytic two-dimension material for the sensor application developed for the first time. In this developed system, uricase interacted with B-N groups by the twodimensional structure of the boron nitride layer. In this way, a unique catalytic structure was created by binding uricase to B-N groups, and this structure was protected by NafionTM. The developed enzyme-based biosensor exhibited a wide linear detection range (5-3000 mu M), and a low limit of detection and quantitation values (0.14 mu M and 0.46 mu M, respectively) for uric acid. Also, the developed system performed good reproducibility, reusability, high selectivity, sensitivity, low Km value, and excellent shelf-life properties. Furthermore, this enzymebased biosensor has successfully identified uric acid in commercial human serum with a high recovery rate. It has promised rapid, high sensitivity and real serum applications to determine the level of uric acid.