The CENP-A nucleosome: where and when it happens during the inner kinetochore's assembly
Creators
- 1. Dokuz Eylul Univ Hlth Campus, Izmir Biomed & Genome Ctr, TR-35330 Izmir, Turkiye
- 2. Univ Claude Bernard Lyon 1, Inst NeuroMyoGene, Lab Physiopathol & Genet Neurone & Muscle PGNM, INSERM U1315, 8 Ave Rockefeller, F-69008 Lyon, France
- 3. Univ Grenoble Alpes, Inst Adv Biosci, INSERM U1209, CNRS,UMR 5309, F-38000 Grenoble, France
- 4. Bulgarian Acad Sci, Roumen Tsanev Inst Mol Biol, Sofia, Bulgaria
- 5. Inst Genet & Biol Mol & Cellulaire IGBMC, UdS, CNRS, INSERM, Illkirch Graffenstaden, France
Description
CENP-A is an essential histone variant that replaces the canonical H3 at the centromeres and marks these regions epigenetically. The CENP-A nucleosome is the specific building block of centromeric chromatin, and it is recognized by CENP-C and CENP-N, two components of the constitutive centromereassociated network (CCAN), the first protein layer of the kinetochore. Recent proposals of the yeast and human (h)CCAN structures position the assembly on exposed DNA, suggesting an elusive spatiotemporal recognition. We summarize the data on the structural organization of the CENP-A nucleosome and the binding of CENP-C and CENP-N. The latter posits an apparent contradiction in engaging the CENP-A nucleosome versus the CCAN. We propose a reconciliatory model for the assembly of CCAN on centromeric chromatin.
Files
bib-21957321-0f41-4298-bc76-00cbc1f9261d.txt
Files
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