Cryopreservation of Deciduous Teeth Originated Mesenchymal Stem Cells with Different Techniques

Objective: Mesenchymal stem cells (MSCs) are not used immediately after isolation in experimental and clinical studies. Traditional freezing and thawing processes may affect the cell viability or immunological properties of MSCs. In this study, the effect of different freezing methods on the viability, differentiation potential, and immunological properties of MSCs from human exfoliated deciduous teeth (SHED) was investigated.Methods: SHED was isolated from SHED from healthy subjects aged between 8-12 years. The third passage cells were frozen with three different freezing solutions containing distinct concentrations of dimethyl sulfoxide, trehalose, ethylene glycol, or PVP40. After thawing, cells were analyzed for cell viability, co-cultured with mononuclear cells, and analyzed for lymphocyte proliferation rate, and cell viability for evaluating immunological properties.Results: Compared to conventional cell freezing methods, the lymphocyte proliferation rate was suppressed, and cell viability ratios were increased with cryoprotectant solutions containing trehalose and ethylene glycol (p<0.05).Conclusion: Cryoprotectant solutions containing trehalose, ethylene glycol, or PVP40 are more suitable for cell freezing.