TUBITAK Destekli Projelerden Çıkan Yayınlar Topluluğu
Yayınlanmış 1 Ocak 2022 | Sürüm v1
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Melatonin in preservation solutions prevents ischemic injury in rat kidneys

Oluşturanlar

  • 1. Marmara Univ, Sch Med, Dept Gen Surg, Istanbul, Turkey
  • 2. Acibadem Mehmet Ali Aydinlar Univ, Sch Med, Dept Histol & Embryol, Istanbul, Turkey
  • 3. Acibadem Mehmet Ali Aydinlar Univ, Sch Med, Dept Med Biochem, Istanbul, Turkey
  • 4. Acibadem Mehmet Ali Aydinlar Univ, Fac Engn, Dept Med Engn, Istanbul, Turkey
  • 5. Istanbul Univ Cerrahpasa, Cerrahpasa Sch Med, Dept Histol & Embryol, Istanbul, Turkey
  • 6. Biruni Univ, Sch Med, Dept Histol & Embryol, Istanbul, Turkey
  • 7. Biruni Univ, Sch Med, Dept Med Biochem, Istanbul, Turkey
  • 8. Mem Hosp, Dept Pathol, Istanbul, Turkey
  • 9. Acibadem Mehmet Ali Aydinlar Univ, Sch Med, Istanbul, Turkey

Açıklama

Transplantation is lifesaving and the most effective treatment for end-stage organ failure. The transplantation success depends on the functional preservation of organs prior to transplantation. Currently, the University of Wisconsin (UW) and histidine-tryptophan-ketoglutarate (HTK) are the most commonly used preservation solutions. Despite intensive efforts, the functional preservation of solid organs prior to transplantation is limited to hours. In this study, we modified the UW solution containing components from both the UW and HTK solutions and analyzed their tissue-protective effect against ischemic injury. The composition of the UW solution was changed by reducing hydroxyethyl starch concentration and adding Histidine/Histidine-HCI which is the main component of HTK solution. Additionally, the preservation solutions were supplemented with melatonin and glucosamine. The protective effects of the preservation solutions were assessed by biochemical and microscopical analysis at 2, 10, 24, and 72 h after preserving the rat kidneys with static cold storage. Lactate dehydrogenase (LDH) activity in preservation solutions was measured at 2, 10, 24, and 72. It was not detectable at 2 h of preservation in all groups and 10 h of preservation in modified UW+melatonin (mUW-m) and modified UW+glucosamine (mUW-g) groups. At the 72nd hour, the lowest LDH activity (0.91 IU/g (0.63-1.17)) was measured in the mUW-m group. In comparison to the UW group, histopathological damage score was low in modified UW (mUW), mUW-m, and mUW-g groups at 10, 24, and 72 hours. The mUW-m solution at low temperature was an effective and suitable solution to protect renal tissue for up to 72 h.

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