Published January 1, 2023
| Version v1
Journal article
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Extraction of weak hydrophobic sulforaphane from broccoli by salting-out assisted hydrophobic deep eutectic solvent extraction
Creators
- 1. Hubei Univ Technol, Bioengn & Food Coll, Key Lab Fermentat Engn, Minist Educ, Wuhan, Hubei, Peoples R China
- 2. Univ Maryland, Dept Chem, Maryland, NY USA
- 3. Johns Hopkins Univ, Div Cardiol, Sch Med, Maryland, NY USA
Description
In order to extract sulforaphane (SFN) from broccoli via green and efficient ways, a novel method based on salting-out assisted deep eutectic solvent (DES) has been developed. Compared to known organic solvent-(such as dichloromethane, ethyl acetate, n-hexane, etc.) based liquid-liquid extraction, this new N8881Cl-based DES method exhibited excellent extraction efficiency for SFN, including a significant improvement due to the salting -out effect of KH2PO4. Under optimal conditions, 97.77 % of SFN was extracted by N8881Cl-EG DES and more than 82.5 % of SFN was recovered by activated carbon from DES. In addition, further studies with Kamlet-Taft pa-rameters and density functional theory showed that the H-bond accepting capacity of hydrophobic DES, the existing vdW interaction, and the electrostatic interaction between N8881Cl-EG DES all contributed to efficient extraction of SFN. This is the first time that the underlying mechanism for SFN extraction by DES was revealed.
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