Published January 1, 2022
| Version v1
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Xenogenic Neural Stem Cell-Derived Extracellular Nanovesicles Modulate Human Mesenchymal Stem Cell Fate and Reconstruct Metabolomic Structure
Creators
- 1. Ankara Univ, Interdisciplinary Res Unit Adv Mat INTRAM, Dept Chem, Fac Sci, TR-06560 Ankara, Turkey
- 2. Hacettepe Univ, Analyt Chem Div, Fac Pharm, TR-06530 Ankara, Turkey
- 3. Ankara Univ, Dept Surg, Fac Vet Med, Ankara, Turkey
- 4. Canakkale Onsekiz Mart Univ, Regenerat Biomat Lab, Dept Bioengn, Engn Fac, TR-17100 Canakkale, Turkey
- 5. Middle East Tech Univ, Dept Biol Sci, Fac Sci, TR-06800 Ankara, Turkey
- 6. Ankara Univ, Dept Biol, Fac Sci, TR-06560 Ankara, Turkey
- 7. Univ Liverpool, Dept Eye & Vis Sci, Inst Life Course & Med Sci, Fac Med, Liverpool L7 8TX, Merseyside, England
Description
Extracellular nanovesicles, particularly exosomes, can deliver their diverse bioactive biomolecular content, including miRNAs, proteins, and lipids, thus providing a context for investigating the capability of exosomes to induce stem cells toward lineage-specific cells and tissue regeneration. In this study, it is demonstrated that rat subventricular zone neural stem cell-derived exosomes (rSVZ-NSCExo) can control neural-lineage specification of human mesenchymal stem cells (hMSCs). Microarray analysis shows that the miRNA content of rSVZ-NSCExo is a faithful representation of rSVZ tissue. Through immunocytochemistry, gene expression, and multi-omics analyses, the capability to use rSVZ-NSCExo to induce hMSCs into a neuroglial or neural stem cell phenotype and genotype in a temporal and dose-dependent manner via multiple signaling pathways is demonstrated. The current study presents a new and innovative strategy to modulate hMSCs fate by harnessing the molecular content of exosomes, thus suggesting future opportunities for rSVZ-NSCExo in nerve tissue regeneration.
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