Collagen/physiologically clotted fibrin-based nanobioscaffold supported with silver nanoparticles: A novel approach

Purpose: In this work, a blend of collagen, physiologically clotted fibrin (PCF), and silver nanoparticles (AgNPs) is used to develop a nanobioscaffold (NBS), for their possible application in wound dressing materials. Methods: The prepared NBS were evaluated using physicochemical, mechanical, and antibacterial properties. The NBS cell viability was demonstrated in a biocompatibility study using the human keratinocyte cell line (HaCaT). Results: The results demonstrated that the NBS had excellent tensile strength (22.15 +/- 0.05 MPa), elongation at break (13.32 +/- 0.09%), and water absorption (97.51 +/- 0.08). The in-vitro study demonstrated its biocompatible nature. NBS exhibited significant antibacterial activity against the Gram-negative and Gram-positive bacteria. Conclusion: The NBS with required mechanical strength, antibacterial activity, and biocompatibility may be tested as a wound material in rats after getting the necessary approval.