ORYX-MRSI: A fully-automated open-source software for proton magnetic resonance spectroscopic imaging data analysis

Proton magnetic resonance spectroscopic imaging (H-1-MRSI) provides a noninvasive, spatially resolved evaluation of brain metabolism. However, there are some limitations of H-1-MRSI preventing its wider use in the clinics, including the spectral quality issues, partial volume effect and chemical shift artifact. Additionally, it is necessary to create metabolite maps for analyzing spectral data along with other MRI modalities. In this study, a MATLAB-based open-source data analysis software for three-dimensional H-1-MRSI, called Oryx-MRSI, which includes modules for visualization of raw H-1-MRSI data and LCModel outputs, chemical shift correction, tissue fraction calculation, metabolite map production, and registration onto standard MNI152 brain atlas while providing automatic spectral quality control, is presented. Oryx-MRSI implements region of interest analysis at brain parcellations defined on MNI152 brain atlas. All generated metabolite maps are stored in NIfTI format. Oryx-MRSI is publicly available at along with six example datasets.