Published January 1, 2022
| Version v1
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Surface plasmon resonance aptasensor for soluble ICAM-1 protein in blood samples
Creators
- 1. Yeditepe Univ, Sch Med, Dept Med Biol, Istanbul, Turkey
- 2. Atilim Univ, Sch Hlth Sci, Dept Nutr & Dietet, Ankara, Turkey
- 3. Konya Food & Agr Univ, KIT ARGEM R&D Ctr, Konya, Turkey
- 4. Yeditepe Univ, Sch Med, Dept Physiol, Istanbul, Turkey
- 5. Atilim Univ, Sch Med, Dept Biol, Ankara, Turkey
- 6. Yeditepe Univ, Sch Med, Dept Biophys, Istanbul, Turkey
Description
Intercellular Adhesion Molecule-1 (ICAM-1) is considered to be a cancer biomarker in the assessment of metastatic potential in patients and an early indicator of atherosclerosis. A labelless biosensor based on the surface plasmon resonance (SPR) signal from the specific affinity interaction of an aptamer and a soluble ICAM-1 protein was developed for blood samples. The developed aptasensor provided real-time information on the concentration of the ICAM-1 protein in blood when integrated to a purification step based on a magnetic pull-down separation. The SPR aptasensor was highly specific with a limit of detection of 1.4/0.2 ng ml(-1), which was achieved through aptamer-functionalized silica-coated magnetic nanoparticles.
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