Isolation of Biological Control Agents and Biotechnological Bacteria from Aquatic Insect Gut Microbiota (Coleoptera: Helophoridae, Hydrophilidae)

Insects have a very large impact among living organisms, both in terms of numbers and efficiency. They are the first actor in pollination, ecological balance and the food chain pyramid. Since gut microbiota plays an important role in the reproduction and spread of living organisms in the food chain, gut microbiota is useful for detection of bacteria to be used as biological control agents. Here, we analysed microbiota (bacteria) of the gut in aquatic Coleoptera (Helephoridae and Hidrophilidae family) by using microbiological and molecular methods. Various conventional methods were used for the complete and accurate identification of bacterial isolates. Gut microbiota bacteria from 17 species of aquatic insects were microbiologically isolated. Using culture-dependent methods, 34 species and 31 different genera were isolated from gut of 17 aquatic insect species (Coleoptera: Helephoridae and Hydrophilidae). Further studies are needed to determine the number and proportion of these bacteria species within total bacterial content for evaluation of biological control and biotechnological uses. As a result of the isolation of bacteria with biotechnological importance, these bacteria will be used repeatedly in biotechnology and biological control.