Analysis of Detailed Chemical and Bioactive Components of Yigilca Honeybee Propolis and Determination of Antioxidant Potential

The bioactive components of propolis differ according to geographical origins, plant resources and bee species. In our research, the chemical components, amino acid and vitamin content, total phenol-flavonoid content and antioxidant activity of Yigilca honey bees propolis collected from 3 different locations in Duzce/Yigilca region were examined. LC-MS/MS method was used to identify the compounds of the propolis samples. Amino acid analysis was also performed with the UFLC system. Vitamin combinations and total phenol-flavonoid contents of propolis were determined using HPLC. Antioxidant activity was measured by FRAP and DPPH methods. LC-MS/MS analysis of propolis samples showed that the amount of Caffeic acid was found to be the highest in all samples with different values. Leucine was detected at the highest rate, while the amino acid determined in the minimum amount was tryptophan. Vitamins B1 and B2 were detected in all samples with different rate except Redifler propolis. The most abundant Vitamin B1 was Redifler sample with the rate of 0.106 mg/100 g and Vitamin B2 was Hosafoglu sample with the rate of 0.130 mg/100 g. Strong antioxidant activity obtained from all samples. As a result, it was concluded that the bioactive components, amino acid and vitamin contents and antioxidant activity of propolis samples different from each other depending on collecting region.