Yayınlanmış 1 Ocak 2010
| Sürüm v1
Dergi makalesi
Açık
Analysis of the Wnt/B-catenin/TCF4 pathway using SAGE, genome-wide microarray and promoter analysis: Identification of BRI3 and HSF2 as novel targets
Oluşturanlar
- 1. Bogazici Univ, Dept Mol Biol & Genet, Istanbul, Turkey
- 2. Bilkent Univ, Dept Mol Biol & Genet, Ankara, Turkey
Açıklama
The Wnt signaling pathway is involved in many differentiation events during embryonic development and can lead to tumor formation after aberrant activation of its components. beta-catenin, a cytoplasmic component, plays a major role in the transduction of canonical Wnt signaling. The aim of this study was to identify novel genes that are regulated by active beta-catenin/TCF signaling in hepatocellular carcinoma-derived Huh7 cells with high (transfected) and low beta-catenin/TCF activities. High TCF activity Huh7 cells led to earlier and larger tumor formation when xenografted into nude mice. SAGE (Serial Analysis of Gene Expression), genome-wide microarray and in silico promoter analysis were performed in parallel, to compare gene expression between low and high beta-catenin/TCF activity clones, and also those that had been rescued from the xenograft tumors. SAGE and genome-wide microarray data were compared and contrasted. BRI3 and HSF2 were identified as novel targets of Wnt/beta-catenin signaling after combined analysis and confirming experiments including qRT-PCR, ChIP, luciferase assay and lithium treatment. (C) 2010 Elsevier Inc. All rights reserved.
Dosyalar
bib-5d8d24b2-3916-4b78-961e-72d422eaec6e.txt
Dosyalar
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