Yayınlanmış 1 Ocak 2010
| Sürüm v1
Dergi makalesi
Açık
A New Approach for in vitro Imaging of Breast Cancer Cells by Anti-Metadherin Targeted PVA-Pyrene
Oluşturanlar
- 1. Ege Univ, Fac Sci, Dept Chem, Div Nucl Chem, TR-35100 Izmir, Turkey
- 2. Ege Univ, Fac Sci, Dept Biochem, TR-35100 Izmir, Turkey
- 3. Istanbul Tech Univ, Dept Chem, Fac Sci & Letters, TR-34469 Istanbul, Turkey
- 4. Adnan Menderes Univ, Sch Med, Dept Infect Dis & Clin Microbiol, TR-09100 Aydin, Turkey
Açıklama
Poly(vinyl alcohol)-pyrene-anti-metadherin (PVA-Py-(Anti-MTDH)), a novel antibody based water soluble probe containing both fluorescent and target sites in the structure for in vitro imaging of breast cancer cells is reported here. Since breast cancer cells have an excess of MDTH protein expressed on the surface, a PVA-Py prepared by "Click chemistry" approach is targeted by Anti-MTDH antibody and applied to the MCF-7 cell line. After characterization, the designed architecture was evaluated in terms of cell incorporation efficiency and compared with a non-targeted structure (PVA-Py). Atomic force microscopy (AFM) and fluorescence microscopy images of cells after incubation of the probe molecules were also obtained to monitor the interaction of the probes with the cancerous
Dosyalar
bib-ae0567db-88fe-4869-b7d0-d21d07fd1f43.txt
Dosyalar
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