Preparation of magnetic horseradish peroxidase-laccase nanoflower for rapid and efficient dye degradation with dual mechanism and cyclic use

Herein, we developed magnetic co-enzymes nanocomposite called mHRP-Lac NF consisting of horseradish peroxidase (HRP), laccase (Lac) incorporated copper II ions (Cu2+) in phosphate buffered saline (PBS) solution and used as an excellent dye degradation agent against a cationic dye, malachite green (MG) and an anionic azo dye acid orange 7 (AO7) under various pH values. Benefiting from the magnetic property, mHRP-Lac NF was repeatedly used for efficient dye degradation.