Published January 1, 2021
| Version v1
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Development of a novel recombinant ELISA for the detection of Crimean-Congo hemorrhagic fever virus IgG antibodies
Creators
- 1. Sivas Cumhuriyet Univ, Dept Infect Dis & Clin Microbiol, Fac Med, Sivas, Turkey
- 2. Ege Univ, Mol Biol Sect, Dept Biol, Fac Sci, Izmir, Turkey
- 3. Ege Univ, Dept Biotechnol, Fac Engn, Izmir, Turkey
- 4. Ege Univ, Dept Parasitol, Fac Med, Izmir, Turkey
- 5. Ege Univ, Dept Bioengn, Fac Engn, Izmir, Turkey
- 6. Erciyes Univ, Dept Med Microbiol, Fac Med, Kayseri, Turkey
- 7. Univ Calif Irvine, Dept Physiol & Biophys, Vaccine Res & Dev Ctr, Irvine, CA 92717 USA
Description
Crimean-Congo hemorrhagic fever (CCHF) is a tick-borne viral infection caused by Crimean-Congo hemorrhagic fever virus (CCHFV). Serological screening of CCHF is important and current ELISA use antigens prepared from virus which is expensive due to requirement of high bio-containment facilities. In this study, we aimed to develop a new recombinant ELISA. For this purpose, CCHFV genome were expressed as 13 proteins in E. coli and among them abundantly purified recombinant Nucleocapsid protein (rNP) and Mucin-like variable domain (rMLD) were used as antigen in ELISA (Rec-ELISA). Rec-ELISA using rNP, rMLD and a combination of both (rNP/rMLD) were probed with acute (n=64; collected between days 1 and 7 after onset of symptoms), convalescent (n=35; collected 8 days after onset of symptoms), consecutive sera (n=25) of confirmed CCHF cases and control sera (n=43). The sensitivity and specificity of Rec-ELISA using rNP/rMLD were 73% and 98% in acute cases and 97% and 98% in convalescent cases. The median interquartile absorbance value to discriminate the acute and convalescent phases of CCHF was significantly higher with ELISA using rNP/rMLD (P<0.0001) compared to rNP (P>0.05) and rMLD (P=0.001). These results indicate that the Rec-ELISA using rNP/rMLD may be very useful to diagnose convalescent CCHF cases especially in field studies.
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