Yayınlanmış 1 Ocak 2021
| Sürüm v1
Dergi makalesi
Açık
Preparation of Low Molecular Weight Heparin from a Remodeled Bovine Intestinal Heparin
Oluşturanlar
- 1. Rensselaer Polytech Inst, Ctr Biotechnol & Interdisciplinary Studies, Troy, NY 12180 USA
- 2. Rensselaer Polytech Inst, Dept Chem & Chem Biol, Ctr Biotechnol & Interdisciplinary Studies, Troy, NY 12180 USA
Açıklama
Bovine intestinal heparins are structurally distinct from porcine intestinal heparins and exhibit lower specific anticoagulant activity (units/mg). The reduced content of N-sulfo, 3-O-sulfo glucosamine, the central and critical residue in heparin's antithrombin III binding site, is responsible for bovine intestinal heparin's reduced activity. Previous studies demonstrate that treatment of bovine intestinal heparin with 3-O-sulfotransferase in the presence of 3'-phosphoadenosine-5'-phosphosulfate afforded remodeled bovine heparin with an enhanced activity reaching the United States Pharmacopeia's requirements. Starting from this remodeled bovine intestinal heparin, we report the preparation of a bovine intestinal low molecular weight heparin having the same structural properties and anti-factor IIa and anti-factor Xa activities of Enoxaparin. Moreover, this bovine intestinal heparin-derived "Enoxaparin" showed comparable platelet factor-4 binding affinity, suggesting that it should exhibit similarly low levels of heparin induced thrombocytopeneia, HIT.
Dosyalar
bib-49b93d3e-4553-49ba-827c-d78820a3acf3.txt
Dosyalar
(273 Bytes)
| Ad | Boyut | Hepisini indir |
|---|---|---|
|
md5:0b1507244feff87f06484f34841f87d5
|
273 Bytes | Ön İzleme İndir |