Published January 1, 2021
| Version v1
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Detection of the effect of polydopamine (PDA)-coated polydimethylsiloxane (PDMS) substrates on the release of H2O2 from a single HeLa cell
- 1. Univ Sci & Technol Beijing, Sch Chem & Biol Engn, Beijing 100083, Peoples R China
- 2. Shenzhen Univ, Sch Biomed Engn, Hlth Sci Ctr, Shenzhen 518037, Peoples R China
- 3. Guangzhou Univ, Ctr Adv Analyt Sci, Sch Chem & Chem Engn, Guangzhou 510006, Peoples R China
Description
Endogenous H2O2 generated by a single HeLa cell that was adhered on the PDA-coated PDMS substrates under 25 mM glucose culture conditions was detected using a home-built photoelectric dual detection platform. With PMA as the stimulus, the cell released a small amount of H2O2 and its mitochondrial membrane potential (MMP) decrease was smaller, compared with that on the PDMS substrates.
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