A novel impedimetric sensor based on CuO(NPs)/polyaniline/murexide composite for cholesterol detection

In this study, we report a novel non-enzymatic cholesterol biosensor based on copper oxide nanoparticles (CuO), polyaniline nanofibers (PANI) and murexide (Mu) modified glassy carbon electrode. The modified electrode was used for the detection of cholesterol. Copper oxide nanoparticles were deposited on the glassy carbon electrode through electrodeposition and electrochemical oxidation followed by electrodeposition of PANI-Mu composite. The prepared GC/CuO(NPs)/PANI/Mu sensor was characterized using electrochemical, optical and morphological methods such as cyclic voltammetry (CV), impedance spectroscopy (EIS), linear sweep voltammetry (LSV), UV-visible and scanning electron microscopy (SEM). The electrochemical response of the elaborated composite matrix for cholesterol detection was evaluated by employing the impedance spectroscopy method. The results were promising as we obtained good analytical performances for cholesterol quantification with high sensitivity of 5575 omega/logM, a wide linear range from 0.5 to 50 mM and a low detection limit of 1 nM.